rabbit polyclonal anti mre 11 antibody  (Novus Biologicals)

Journal: Frontiers in Cell and Developmental Biology

Article Title: MRE11 orchestrates porcine oocyte meiotic progression by modulating the spindle assembly checkpoint

doi: 10.3389/fcell.2025.1635110

Figure Lengend Snippet: Subcellular localization and protein expression of Mre11 during porcine meiotic maturation. (A) Representative immunofluorescence images showing the localization of endogenous Mre11 in porcine oocytes at different developmental stages. Oocytes were immunostained with an anti-Mre11 antibody and counterstained with Hoechst. Scale bar, 2.5 μm. (B) Representative image showing the co-localization of Mre11 with kinetochores in porcine oocytes at the pro-metaphase I (Pro-M I) stage. Oocytes were immunostained with anti-Mre11 and CREST antibodies, and counterstained with Hoechst. Scale bar, 2.5 μm.

Article Snippet: Rabbit polyclonal anti-MRE11 antibody was purchased from Proteintech Group (Rosemont, IL, United States; Cat# 16370-1-AP); rabbit monoclonal anti-BUBR1 antibody was purchased from Abcam (Cambridge, United Kingdom, United States; Cat# ab133699); Mouse anti-α-tubulin-FITC antibody (Cat# F2168), mouse monoclonal anti-acetylation-α-tubulin antibody (T7451), peanut agglutinin (PNA)-FITC (L7381) and DAPI (D9542) were purchased from Sigma (Aldrich, St Louis, MO, United States); Rabbit polyclonal anti-γ-H2AX antibody (Cat# ab26350) was purchased from Abcam (Cambridge, United Kingdom); TRITC Phalloidin TRITC antibody (Cat# MX4405) was purchased from Maokang Biotechnology (Shanghai, China); Furthermore, goat anti-mouse IgG Alexa Fluor 488 antibody (Cat# A11029), donkey anti-Sheep IgG Alexa Fluor 594 antibody (Cat# A11016) were purchased from Thermo Fisher (Waltham, MA, United States). human anti-centromere antibody was purchased from Antibodies Incorporated (Davis, CA, United States; Cat# CA95617); rabbit monoclonal anti-GAPDH antibody was purchased from Cell Signaling Technology (Danvers, MA, United States; Cat# 2118).

Techniques: Expressing, Immunofluorescence

Journal: Frontiers in Cell and Developmental Biology

Article Title: MRE11 orchestrates porcine oocyte meiotic progression by modulating the spindle assembly checkpoint

doi: 10.3389/fcell.2025.1635110

Figure Lengend Snippet: Effect of Mre11 inhibition on porcine oocyte meiotic progression. (A) Representative images of cumulus cell expansion and polar body extrusion in control and Mre11-inhibited oocytes cultured in vitro for 44 h. Oocytes were denuded after culture to observe polar body extrusion. COCs: cumulus-oocyte complexes; DOs: denuded oocytes. Scale bars, 500 μm (a, e); 200 μm (b, f); 250 μm (c, g); 30 μm (d, h). (B) The rate of polar body extrusion was recorded in control and Mre11-inhibited groups treated with different concentrations (20 μM, 40 μM, 60 μM, and 100 μM) after 44 h of culture. (C) Representative images showing chromosome morphology at different developmental stages of oocyte maturation. DNA was counterstained with propidium iodide (PI). Scale bar, 5 μm. (D) The percentage of oocytes at different developmental stages was quantified in control and Mre11-inhibited groups. Data in panels (B) and (D) are presented as mean percentages (mean ± SEM) from at least three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.

Article Snippet: Rabbit polyclonal anti-MRE11 antibody was purchased from Proteintech Group (Rosemont, IL, United States; Cat# 16370-1-AP); rabbit monoclonal anti-BUBR1 antibody was purchased from Abcam (Cambridge, United Kingdom, United States; Cat# ab133699); Mouse anti-α-tubulin-FITC antibody (Cat# F2168), mouse monoclonal anti-acetylation-α-tubulin antibody (T7451), peanut agglutinin (PNA)-FITC (L7381) and DAPI (D9542) were purchased from Sigma (Aldrich, St Louis, MO, United States); Rabbit polyclonal anti-γ-H2AX antibody (Cat# ab26350) was purchased from Abcam (Cambridge, United Kingdom); TRITC Phalloidin TRITC antibody (Cat# MX4405) was purchased from Maokang Biotechnology (Shanghai, China); Furthermore, goat anti-mouse IgG Alexa Fluor 488 antibody (Cat# A11029), donkey anti-Sheep IgG Alexa Fluor 594 antibody (Cat# A11016) were purchased from Thermo Fisher (Waltham, MA, United States). human anti-centromere antibody was purchased from Antibodies Incorporated (Davis, CA, United States; Cat# CA95617); rabbit monoclonal anti-GAPDH antibody was purchased from Cell Signaling Technology (Danvers, MA, United States; Cat# 2118).

Techniques: Inhibition, Control, Cell Culture, In Vitro

Journal: Frontiers in Cell and Developmental Biology

Article Title: MRE11 orchestrates porcine oocyte meiotic progression by modulating the spindle assembly checkpoint

doi: 10.3389/fcell.2025.1635110

Figure Lengend Snippet: Effect of Mre11 inhibition on BubR1 localization in porcine oocytes. (A) Representative images showing the localization of BubR1 at the metaphase I stage in control and Mre11-inhibited oocytes. Scale bar, 5 μm. (B) Quantitative analysis of BubR1 fluorescence intensity in control and Mre11-inhibited oocytes. Data are presented as the mean percentage (mean ± SEM) from at least three independent experiments. **P < 0.001.

Article Snippet: Rabbit polyclonal anti-MRE11 antibody was purchased from Proteintech Group (Rosemont, IL, United States; Cat# 16370-1-AP); rabbit monoclonal anti-BUBR1 antibody was purchased from Abcam (Cambridge, United Kingdom, United States; Cat# ab133699); Mouse anti-α-tubulin-FITC antibody (Cat# F2168), mouse monoclonal anti-acetylation-α-tubulin antibody (T7451), peanut agglutinin (PNA)-FITC (L7381) and DAPI (D9542) were purchased from Sigma (Aldrich, St Louis, MO, United States); Rabbit polyclonal anti-γ-H2AX antibody (Cat# ab26350) was purchased from Abcam (Cambridge, United Kingdom); TRITC Phalloidin TRITC antibody (Cat# MX4405) was purchased from Maokang Biotechnology (Shanghai, China); Furthermore, goat anti-mouse IgG Alexa Fluor 488 antibody (Cat# A11029), donkey anti-Sheep IgG Alexa Fluor 594 antibody (Cat# A11016) were purchased from Thermo Fisher (Waltham, MA, United States). human anti-centromere antibody was purchased from Antibodies Incorporated (Davis, CA, United States; Cat# CA95617); rabbit monoclonal anti-GAPDH antibody was purchased from Cell Signaling Technology (Danvers, MA, United States; Cat# 2118).

Techniques: Inhibition, Control, Fluorescence

Journal: Frontiers in Cell and Developmental Biology

Article Title: MRE11 orchestrates porcine oocyte meiotic progression by modulating the spindle assembly checkpoint

doi: 10.3389/fcell.2025.1635110

Figure Lengend Snippet: Effect of Mre11 inhibition on spindle assembly and chromosome alignment in porcine oocytes. (A) Representative images depicting spindle morphologies and chromosome alignment in control and Mre11-inhibited oocytes. Metaphase I (M I) oocytes were immunostained with anti-α-tubulin-FITC antibody to visualize spindles and counterstained with propidium iodide (PI) to visualize chromosomes. Scale bar, 5 μm. (B) The frequency of aberrant spindles was recorded in control and Mre11-inhibited oocytes. Data in panel (B) are presented as mean percentages (mean ± SEM) from at least three independent experiments.

Article Snippet: Rabbit polyclonal anti-MRE11 antibody was purchased from Proteintech Group (Rosemont, IL, United States; Cat# 16370-1-AP); rabbit monoclonal anti-BUBR1 antibody was purchased from Abcam (Cambridge, United Kingdom, United States; Cat# ab133699); Mouse anti-α-tubulin-FITC antibody (Cat# F2168), mouse monoclonal anti-acetylation-α-tubulin antibody (T7451), peanut agglutinin (PNA)-FITC (L7381) and DAPI (D9542) were purchased from Sigma (Aldrich, St Louis, MO, United States); Rabbit polyclonal anti-γ-H2AX antibody (Cat# ab26350) was purchased from Abcam (Cambridge, United Kingdom); TRITC Phalloidin TRITC antibody (Cat# MX4405) was purchased from Maokang Biotechnology (Shanghai, China); Furthermore, goat anti-mouse IgG Alexa Fluor 488 antibody (Cat# A11029), donkey anti-Sheep IgG Alexa Fluor 594 antibody (Cat# A11016) were purchased from Thermo Fisher (Waltham, MA, United States). human anti-centromere antibody was purchased from Antibodies Incorporated (Davis, CA, United States; Cat# CA95617); rabbit monoclonal anti-GAPDH antibody was purchased from Cell Signaling Technology (Danvers, MA, United States; Cat# 2118).

Techniques: Inhibition, Control

Journal: Frontiers in Cell and Developmental Biology

Article Title: MRE11 orchestrates porcine oocyte meiotic progression by modulating the spindle assembly checkpoint

doi: 10.3389/fcell.2025.1635110

Figure Lengend Snippet: Effect of Mre11 inhibition on the acetylation level of α-tubulin in porcine oocytes. (A) Representative images of microtubule fibers before and after nocodazole treatment in control and Mre11-inhibited oocytes. Oocytes were immunostained with an anti-α-tubulin-FITC antibody to visualize microtubules and counterstained with propidium iodide (PI) to visualize chromosomes. Scale bar, 10 μm. (B) Representative images showing the acetylation of α-tubulin in control and Mre11-inhibited oocytes. Oocytes were immunostained with an anti-acetyl-α-tubulin (Lys-40) antibody to assess the acetylation level of α-tubulin. Scale bar, 5 μm. (C) The fluorescence intensity of acetylated α-tubulin was quantified in control and Mre11-inhibited oocytes. Data in panel (C) are presented as mean percentages (mean ± SEM) from at least three independent experiments. ***P < 0.001.

Article Snippet: Rabbit polyclonal anti-MRE11 antibody was purchased from Proteintech Group (Rosemont, IL, United States; Cat# 16370-1-AP); rabbit monoclonal anti-BUBR1 antibody was purchased from Abcam (Cambridge, United Kingdom, United States; Cat# ab133699); Mouse anti-α-tubulin-FITC antibody (Cat# F2168), mouse monoclonal anti-acetylation-α-tubulin antibody (T7451), peanut agglutinin (PNA)-FITC (L7381) and DAPI (D9542) were purchased from Sigma (Aldrich, St Louis, MO, United States); Rabbit polyclonal anti-γ-H2AX antibody (Cat# ab26350) was purchased from Abcam (Cambridge, United Kingdom); TRITC Phalloidin TRITC antibody (Cat# MX4405) was purchased from Maokang Biotechnology (Shanghai, China); Furthermore, goat anti-mouse IgG Alexa Fluor 488 antibody (Cat# A11029), donkey anti-Sheep IgG Alexa Fluor 594 antibody (Cat# A11016) were purchased from Thermo Fisher (Waltham, MA, United States). human anti-centromere antibody was purchased from Antibodies Incorporated (Davis, CA, United States; Cat# CA95617); rabbit monoclonal anti-GAPDH antibody was purchased from Cell Signaling Technology (Danvers, MA, United States; Cat# 2118).

Techniques: Inhibition, Control, Fluorescence

Journal: Frontiers in Cell and Developmental Biology

Article Title: MRE11 orchestrates porcine oocyte meiotic progression by modulating the spindle assembly checkpoint

doi: 10.3389/fcell.2025.1635110

Figure Lengend Snippet: Effect of Mre11 inhibition on cytoplasmic actin assembly in porcine oocytes. (A) Representative images showing the distribution of actin filaments at the oocyte cortex and cytoplasm in the control and Mre11-inhibited groups. Red indicates F-actin. Scale bar, 20 μm. (B) Quantification of fluorescence intensity of F-actin at the cortex in the control and Mre11-inhibited groups. (C) Quantification of fluorescence intensity of F-actin in the cytoplasm in the control and Mre11-inhibited groups. (D) Quantitative analysis of F-actin fluorescence intensities at the cortex and in the cytoplasm. Data in panels (B–D) are presented as mean percentages (mean ± SEM) from at least three independent experiments. ***P < 0.001.

Article Snippet: Rabbit polyclonal anti-MRE11 antibody was purchased from Proteintech Group (Rosemont, IL, United States; Cat# 16370-1-AP); rabbit monoclonal anti-BUBR1 antibody was purchased from Abcam (Cambridge, United Kingdom, United States; Cat# ab133699); Mouse anti-α-tubulin-FITC antibody (Cat# F2168), mouse monoclonal anti-acetylation-α-tubulin antibody (T7451), peanut agglutinin (PNA)-FITC (L7381) and DAPI (D9542) were purchased from Sigma (Aldrich, St Louis, MO, United States); Rabbit polyclonal anti-γ-H2AX antibody (Cat# ab26350) was purchased from Abcam (Cambridge, United Kingdom); TRITC Phalloidin TRITC antibody (Cat# MX4405) was purchased from Maokang Biotechnology (Shanghai, China); Furthermore, goat anti-mouse IgG Alexa Fluor 488 antibody (Cat# A11029), donkey anti-Sheep IgG Alexa Fluor 594 antibody (Cat# A11016) were purchased from Thermo Fisher (Waltham, MA, United States). human anti-centromere antibody was purchased from Antibodies Incorporated (Davis, CA, United States; Cat# CA95617); rabbit monoclonal anti-GAPDH antibody was purchased from Cell Signaling Technology (Danvers, MA, United States; Cat# 2118).

Techniques: Inhibition, Control, Fluorescence

Journal: Frontiers in Cell and Developmental Biology

Article Title: MRE11 orchestrates porcine oocyte meiotic progression by modulating the spindle assembly checkpoint

doi: 10.3389/fcell.2025.1635110

Figure Lengend Snippet: Effect of Mre11 inhibition on meiotic spindle positioning and the formation of actin caps in porcine oocytes. (A) Representative images showing spindle positioning in control and Mre11-inhibited oocytes. (B) To quantify spindle position after Mre11 inhibition, D is defined as the distance from the proximal end of the spindle pole to the cortex, while L is defined as the distance from the distal end of the spindle pole to the cortex. (C) The D/L ratio in the Mre11-inhibited group was significantly higher than that in the control group. (D) Formation of actin caps and CGFDs. After Mre11 inhibition, the actin cap over a chromosome was disrupted, and Mre11 inhibition also affected CGFD formation. Red indicates actin; green indicates CGs; blue indicates chromatin. Scale bar, 20 μm.

Article Snippet: Rabbit polyclonal anti-MRE11 antibody was purchased from Proteintech Group (Rosemont, IL, United States; Cat# 16370-1-AP); rabbit monoclonal anti-BUBR1 antibody was purchased from Abcam (Cambridge, United Kingdom, United States; Cat# ab133699); Mouse anti-α-tubulin-FITC antibody (Cat# F2168), mouse monoclonal anti-acetylation-α-tubulin antibody (T7451), peanut agglutinin (PNA)-FITC (L7381) and DAPI (D9542) were purchased from Sigma (Aldrich, St Louis, MO, United States); Rabbit polyclonal anti-γ-H2AX antibody (Cat# ab26350) was purchased from Abcam (Cambridge, United Kingdom); TRITC Phalloidin TRITC antibody (Cat# MX4405) was purchased from Maokang Biotechnology (Shanghai, China); Furthermore, goat anti-mouse IgG Alexa Fluor 488 antibody (Cat# A11029), donkey anti-Sheep IgG Alexa Fluor 594 antibody (Cat# A11016) were purchased from Thermo Fisher (Waltham, MA, United States). human anti-centromere antibody was purchased from Antibodies Incorporated (Davis, CA, United States; Cat# CA95617); rabbit monoclonal anti-GAPDH antibody was purchased from Cell Signaling Technology (Danvers, MA, United States; Cat# 2118).

Techniques: Inhibition, Control

Journal: Frontiers in Cell and Developmental Biology

Article Title: MRE11 orchestrates porcine oocyte meiotic progression by modulating the spindle assembly checkpoint

doi: 10.3389/fcell.2025.1635110

Figure Lengend Snippet: Effect of Mre11 inhibition on DNA damage levels in porcine oocytes. (A) Representative images showing DNA damage in control and Mre11-inhibited oocytes. Oocytes were immunostained with an anti-γH2A.X antibody and counterstained with Hoechst. Scale bar, 5 μm. (B) Quantification of γH2A.X fluorescence intensity in control and Mre11-inhibited oocytes. Data are presented as mean percentages (mean ± SEM) from at least three independent experiments. ***P < 0.001.

Article Snippet: Rabbit polyclonal anti-MRE11 antibody was purchased from Proteintech Group (Rosemont, IL, United States; Cat# 16370-1-AP); rabbit monoclonal anti-BUBR1 antibody was purchased from Abcam (Cambridge, United Kingdom, United States; Cat# ab133699); Mouse anti-α-tubulin-FITC antibody (Cat# F2168), mouse monoclonal anti-acetylation-α-tubulin antibody (T7451), peanut agglutinin (PNA)-FITC (L7381) and DAPI (D9542) were purchased from Sigma (Aldrich, St Louis, MO, United States); Rabbit polyclonal anti-γ-H2AX antibody (Cat# ab26350) was purchased from Abcam (Cambridge, United Kingdom); TRITC Phalloidin TRITC antibody (Cat# MX4405) was purchased from Maokang Biotechnology (Shanghai, China); Furthermore, goat anti-mouse IgG Alexa Fluor 488 antibody (Cat# A11029), donkey anti-Sheep IgG Alexa Fluor 594 antibody (Cat# A11016) were purchased from Thermo Fisher (Waltham, MA, United States). human anti-centromere antibody was purchased from Antibodies Incorporated (Davis, CA, United States; Cat# CA95617); rabbit monoclonal anti-GAPDH antibody was purchased from Cell Signaling Technology (Danvers, MA, United States; Cat# 2118).

Techniques: Inhibition, Control, Fluorescence

Journal: PLOS Pathogens

Article Title: Hepatitis B virus hijacks MRE11–RAD50–NBS1 complex to form its minichromosome

doi: 10.1371/journal.ppat.1012824

Figure Lengend Snippet: Antibodies used for western blotting.

Article Snippet: Anti-MRE11 Rabbit Polyclonal Antibody , ABclonal, Wuhan, China , Cat# A2559.

Techniques: Western Blot

Journal: PLOS Pathogens

Article Title: Hepatitis B virus hijacks MRE11–RAD50–NBS1 complex to form its minichromosome

doi: 10.1371/journal.ppat.1012824

Figure Lengend Snippet: (A) Schematic diagram of biotinylated HBV rcDNA pull-down and mass spectrometry assay. (B) GO analysis of target proteins were performed using Metascape. Bar graph of enriched terms across input gene lists, colored by p-values. (C) Protein-protein interaction network and molecular complex detection (MCODE) components identified in the gene lists. The three components of MRN complex: MRE11, RAD50 and NBS1 were labeled in red. The interaction between HBV rcDNA and MRN complex was further confirmed by western blotting (D) and chromatin immunoprecipitation assay (E), the numbers below the blot indicate the arbitrary units from the densitometry analysis of indicated bands. ***: p <0.001.

Article Snippet: Anti-MRE11 Rabbit Polyclonal Antibody , ABclonal, Wuhan, China , Cat# A2559.

Techniques: Mass Spectrometry, Labeling, Western Blot, Chromatin Immunoprecipitation

Journal: PLOS Pathogens

Article Title: Hepatitis B virus hijacks MRE11–RAD50–NBS1 complex to form its minichromosome

doi: 10.1371/journal.ppat.1012824

Figure Lengend Snippet: (A and B) rcDNA-I produced in vitro and virion-rcDNA (B) extracted from HBV virions were incubated with purified MRE11 at 37°C for 30 min. 300 μM indicated inhibitors were used. The numbers below the blot indicate the arbitrary units from the densitometry analysis of rcDNA bands. M: marker. *: degraded DNA.

Article Snippet: Anti-MRE11 Rabbit Polyclonal Antibody , ABclonal, Wuhan, China , Cat# A2559.

Techniques: Produced, In Vitro, Incubation, Purification, Marker

Journal: Surgery today

Article Title: Carcinogenic risk in the biliary epithelium of children with congenital biliary dilatation via the DNA damage repair pathway.

doi: 10.1007/s00595-023-02664-2

Figure Lengend Snippet: Fig. 2 MRE11 expression in gallbladder epithelium (all data are shown as medians)

Article Snippet: Immunohistochemical staining was performed on 4-μm-thick sections using a rabbit monoclonal anti-γH2AX antibody (Cell Signaling Technology, Inc., Danvers, MA, USA; #9718; diluted 1:240 in phosphate-buffered saline [PBS]), a rabbit polyclonal anti-Mre11 antibody (Cell Signaling Technology, Inc.; #4895; diluted 1:500 in PBS), and a mouse monoclonal anti-Ku70 antibody (Santa Cruz Biotechnology, Inc., Dallas, TX, USA; sc-17789; diluted 1:50 in PBS).

Techniques: Expressing

Journal: Surgery today

Article Title: Carcinogenic risk in the biliary epithelium of children with congenital biliary dilatation via the DNA damage repair pathway.

doi: 10.1007/s00595-023-02664-2

Figure Lengend Snippet: Fig. 5 MRE11 expression in bile duct epithelium (all data are shown as medians)

Article Snippet: Immunohistochemical staining was performed on 4-μm-thick sections using a rabbit monoclonal anti-γH2AX antibody (Cell Signaling Technology, Inc., Danvers, MA, USA; #9718; diluted 1:240 in phosphate-buffered saline [PBS]), a rabbit polyclonal anti-Mre11 antibody (Cell Signaling Technology, Inc.; #4895; diluted 1:500 in PBS), and a mouse monoclonal anti-Ku70 antibody (Santa Cruz Biotechnology, Inc., Dallas, TX, USA; sc-17789; diluted 1:50 in PBS).

Techniques: Expressing

Journal: Cancers

Article Title: Targeting BRF2 in Cancer Using Repurposed Drugs

doi: 10.3390/cancers13153778

Figure Lengend Snippet: List of antibodies used in this paper.

Article Snippet: Polyclonal Rabbit anti-MRE11 (WB-1:1000) , Becton Dickinson Biosciences, USA.

Techniques:

Journal: Molecular cell

Article Title: A mechanism to minimize errors during non-homologous end joining

doi: 10.1016/j.molcel.2019.11.018

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit polyclonal antibody against X. laevis Mre11 was raised against the peptide CDDEEDFDPFKKSGPSRRGRR by Bethyl Laboratories.

Techniques: Recombinant, Expressing, Software